簡麗鳳[ 副教授 ]

生物能量研究室
辦公室電話: (04) 22840416 # 609
實驗室電話: (04) 22840416 # 608
傳   真: (04) 22874740
實驗室位置: 生命科學大樓 606 室
最高學歷: 英國劍橋大學生物化學博士
專長領域: 生物能量代謝機制之調控分析  
電子信箱: lfchien@dragon.nchu.edu.tw
實驗室網站:  
 
年份 標題
2014 2014 指導台中一中同學中區科展特優
 
畢業學校 主修學門系所 學位 時期
英國劍橋大學 生物化學系 博士 1992 - 1996
清華大學 輻射生物研究所 碩士 1988 - 1990
中原大學 化學系 學士 1982 - 1986
 
服務機關 服務部門 職稱 時期
中興大學 生命科學系 副教授 2011.08-迄今
中興大學 生命科學系 助理教授 2002 - 2011
中興大學 植物系 助理教授 2000 - 2002
以色列本古里昂大學 布勞斯丁沙漠研究所 博士後研究員 1999 - 2000
東華大學 生物技術研究所 副研究員 1998 - 1999
中央研究院 植物/分子生物研究所 博士後研究員 1996 - 1998
 
研究興趣
1.Control of energy transduction
2.Photobiological H2 production in green algae
3.Alternative pathways of bamboo mitochondrial energy metabolism

研究主題
1 Generation of a zero-CO2-emission green alga factory for photo-biological H2 production.
2 Functional expression of foreign genes in green alga Chlorella.
3 Analysis of energy metabolism in bamboo mitochondria.

目前研究計畫
1 藻類研發平台:小球藻產氫路徑之分析 (中興NCHU 101,102,103,104,105)
2 有效率的太陽能-to-生物產氫:改造小球藻產氫酶的H2 或O2 channels
(國科會)
3 太陽能-to-生物氫能: 以基因工程技術增進小球藻產氫效能 (國科會)
4 建構無CO2之光生化產H2綠色工場 : 於綠藻去O2突變株過度表現產氫? (國科會)
 
學期 科目名稱 學分 開設班級
下學期 植物生理學
下學期 生物物理化學
下學期 生物物理特論
上學期 普通植物學
上學期 植物生理學
上學期 生物能量學
上學期 生物光譜學
博士班
陳秀惠(共同指導)、周少華、林昕笛

碩士班
徐晁偉、林彥儒、陳正修

大學部
林昱廷

研究助理

博士後研究

畢業生
 
A.期刊論文
 
1.Chen YS, Chesson P, Wu HW, Pao SH, Liu JW, Chien LF, Yong JW, Sheue CR*. 2016/12 Leaf structure affects a plant's appearance: combined multiple-mechanisms intensify remarkable foliar variegation.,J Plant Res.. (SCI)
 
2.Hsiu-Hui Chen, Tung-Chuan Yang, Tsung-Hsien Lee, Chun-Chia Huang, Maw-Sheng Lee*, Lee-Feng Chien* 2016/08 Woman age limits the improvable effect of reduced oxygen concentration (5% O2) on human embryo quality and pregnancy potential,International Journal of Clinical and Experimental Medicine.. vol.9, pp.16459-16467. (SCI)
 
3.Lin HD, Liu BH, Kuo TT, Tsai HC, Feng TY, Huang CC*, Chien LF*. 2013 Knockdown of PsbO leads to induction of HydA and production of photobiological H2 in the green alga Chlorella sp. DT., Bioresource Technology.(IF= 4.494; Rank: 13/89 [14.6%]/ Energy & Fuels/ 2014) . vol. 143 , pp.154-162. (SCI)
 
4.Chien LF,*, Kuo TT, Liu BH, Lin HD,Feng TY, Huang CC*. 2012 Solar-to-bioH2 production enhanced by homologous overexpression of hydrogenase in green alga Chlorella sp. DT.,Internation Journal of Hydrogen Energy (IF=3.313; Rank:25/89 [28.0%]/ Energy & Fuels/ 2014). vol.37, pp.17738-17748. (SCI)
 
5.Sheue CR*, Pao SH, Chien LF,Chesson P, Peng CI. 2012 Natural foliar variegation without costs? The case of Begonia,Annals of Botany (London) (IF=3.654; Rank:26/204 [12.7%]/ Plant Science/ 2014). vol.109 , pp.1065-1074. (SCI)
 
6.Weng JH*, Chien LF, Jiang CY, Shih FC, Chen HY. 2011 A comparison between yellow-green and green cultivars of four vegetable species in pigments, ascorbate, photosynthesis, energy dissipation, and photoinhibition.,Photosynthetica (IF= 1.409; Rank: 99/204 [48.5%] /Plant Sciences / 2014) . vol.49, pp.361-370. (SCI)
 
7.Chien LF*, Wu YC, Chen HP. 2011 Mitochondrial energy metabolism in young bamboo rhizomes from Bambusa oldhamii and Phyllostachys edulis during shooting stage.,Plant Physiol. Biochem.(IF=2.756; Rank:44/204 [21.6%]/ Plant Sciences/ 2014). vol.49, pp.449-457. (SCI)
 
8.Shieh GJ, Chien LF*, Pan RL 2011 The extrinsic proteins of oxygen-evolving complex in marine diatom Cylindrotheca fusiformis.,Botanical Studies (IF=0.783; Rank:145/204 [71.1%] /Plant Science/ 2014). vol.52, pp.161-171. (SCI)
 
9.Chien LF*, Vonshak A. 2011 Enzymatic antioxidant response to low temperature acclimation in the cyanobacterium Arthrospira platensis.,Journal of Applied Phycology (IF=2.559; Rank:18/103 [17.5%], Marine & Freshwater/ 2014). vol.23, pp.887-894. (SCI)
 
10.Hsiao Y-Y, Pan Y-J, Hsu S-H, Huang Y-T, Liu T-H, Lee C-H, Lee C-H, Liu P-F, Chang W-C, Wang Y-K, Chien L-F, Pan R-L* 2007 Functional roles of arginine residues in mung bean vacuolar H+-pyrophosphatase. ,Biochim. Biophys. Acta -Bioenergetics (IF=5.353; Rank: 11/74 [14.9%] / Biophysics/ 2014) . vol.1767, pp.965-973. (SCI)
 
11.Huang C-C, Chen M-W, Hsieh J-L, Lin W-H, Chen P-C, Chien L-F*. 2006 Expression of mercuric reductase from Bacillus megaterium MB1 in eukaryotic microalga Chlorella sp DT,Appl. Microb. Biotechnol. (IF=3.337; Rank: 39/163 [23.9%]/ Biotechnology & Applied Microbiology/ 2014). vol.72, pp.197-205. (SCI)
 
12.Lee SC, Chien LF*, Van RC, Hsiao YY, Hong JL, Pan RL. 2006 Radiation inactivation analysis of thylakoid protein kinase systems in light and in darkness,Photosynthetica. (IF= 1.409; Rank: 99/204 [48.5%] / Plant Sciences / 2014). vol.44, pp.116-124. (SCI)
B.研討會論文
 
1.Ting-Ting Kuo, Yen-Ju Lin, Lee-Feng Chien* 2016/10 Invited speaker: Clean energy H2 photobiologically produced by green algal Chlorella sp. DT mutants with overexpressed hydrogenase or ferredoxin. Abstract.,2016光合作用與植物生理研討會. Taipei. (其他)
 
2.Ting-Ting Kuo, Yen-Ju Lin, Lee-Feng Chien* 2016/08 Invited speaker: Clean bioenergy H2 photobiologically produced by green algal Chlorella sp. DT mutants with overexpressed hydrogenase or ferredoxin.,6th International Symposium on Energy Challenges and Mechanics. Towards a Big Picture.. Inverness, Scotland, UK. (其他)
 
3.Syu JW, Yang DW, Chien LF. 2014 The hydrogen production of high oxygen tolerance hydrogenase mutant of Cholrella sp. DT. Poster Abstract: 2014 Plant Science Annual Meeting & Symposium,13-14 December 2014, Taipei, Taiwan.
 
4.Lin YJ, Yang DW, Chien LF 2014 Construction of p121-crfd-gfp vector for overexpressing ferredoxin 1 in Chlorella sp. DT hydrogenase mutants. Poster Abstract:2014 Plant Science Annual Meeting & Symposium,13-14 December 2014,Taipei, Taiwan..
 
5.Kuo TT, Liu BH, Lin HD, Feng TY, Huang CC, Chien LF. 2014 Overexpression of HydA or knockdown of PsbO lead to the increase in the photobiological H2 production in green alga Chlorella sp. DT. Poster Abstract: The 15th IUBMB-24th FAOBMB-TSBMB Conference. 21-26th Oct, 2014, Taipei, Taiwan.
 
6. Hsu CW, Chen LF 2013 Expression of mitochondrial-encoded Nad2 and Nad5 subunits of bamboo Phyllostachys edulis mitochondrial NADH:ubiquinone oxidoreductase in Saccharomyces cerevisiae. Poster Abstract:,The 28th Joint Annual Conference of Biomedical Sciences, 16-17 March, 2013. Taipei, Taiwan.
 
7.Yang DW, Chen LF 2013 Green alga Chlorella hydrogenase mutants that have high O2 tolerance. Poster Abstract: The 28th Joint Annual Conference of Biomedical Sciences,16-17 March, 2013, Taipei, Taiwan. P726.
 
8.Lin HD, Liu BH, Kuo TT, Chien LF*. 2012 Invited speaker: (IFIB-2012 Biorefinery Technology 7) Knockdown of PsbO leads to the induction of HydA and the production of photobiological H2 in green alga Chlorella sp. DT, Abstract: IFIB-2012 5th International Conference on Industrial Bioprocess in Taiwan 7-10 October, 2012.
 
9.Hsieh CH, Chein LF 2011 Expression of bamboo mitochondrial-encoded Nad1 subunit in Saccharomyces cerevisiae,The 26th Joint Annual Conference of Biomedical Sciences. Taipei, March, 2011. #P1. .
 
10.Chien LF, Lin CD, Kou TT 2010 Production of H2 by solar-driven pathway as electrons from photosynthetic electron transport chain being transferring to hydrogenase,Satellite Meeting of the 15th International Photosynthesis Congress, ISPR, Singapore, August, . pp.38.
 
11.Liu BH, Chien LF 2010 Construction of the RNA interference Vectors to knockdown PsbO protein in Chlorella sp DT,The 25th Joint Annual Conference of Biomedical Sciences. Taipei, March, pp.434.
 
12.Kou TT, Chien LF 2010 Homologous overexpression of hydrogenase in Chlorella sp. DT to enhance hydrogen production,The 25th Joint Annual Conference of Biomedical Sciences. Taipei, March, 2010, pp.449.
 
13.Lin HD, Chien LF 2009 Using RNA interference to generate D1-deficient or PsbO-deficient Chlorella sp DT for hydrogen production,The 24th Joint Annual Conference of Biomedical Sciences. Taipei, March, , pp.735.
 
14.Chien LF, Wu YC, Ko GC 2009 Mitochondrial proteome in young bamboo rhizome from Bambusa oldhamii and Phyllostachys edulis,Abstract: Plant Biology . Hawaii, July, pp.45021.
 
15.Chiu CY, Chien LF 2009 Proteomic analysis of merA transgenic Chlorella by IEF/SDS-PAGE,The 24th Joint Annual Conference of Biomedical Sciences. Taipei, March, pp.740.
 
16.Wang SH, Chien LF 2009 Expression and characterization of bamboo mitochondrial-encoded Cox2 subunit in yeast,The 24th Joint Annual Conference of Biomedical Sciences. Taipei, March, pp.736.
 
17.Chien LF, Chen HP, Wu YC 2008 Bamboo mitochondrial energy metabolic pathways in Bambusa oldhamii and PhyllostBiochim achys edulis during rapid shooting stage,Acta-Bioenergetics 1777: S98. #S13.41. 15th European Bioenergetics Conference, Dublin, Ireland, 19-24th July 2008.
 
18.Tsai HC, Hsu YH, Chien LF 2007 Genomic analysis of plant mitochondrial nd1 and nd5 subunits of nadh:ubiquinone oxidoreductase,The 22nd Joint Annual Conference of Biomedical Sciences. Taipei, March, pp.355.
 
19.Huang HS, Chang HY, Chen HP, Lau YY, Wu GW, Shi SH, Tsai HC, Chien LF 2007 Variation in photosynthesis, respiration, and superoxide dismutase activity in Bolboschoenus planicu ,The 22nd Joint Annual Conference of Biomedical Sciences. Taipei, March, pp.988.
 
20.Chien LF, Chen MW, Huang CC 2006 Mercury phytoremediation: functional expression of mercuric reductase from Bacillus megaterium MB1 i Abstract:Plant Biology 2006,5-9 August, Boston. pp.06024.
 
21.Wu YC, Chien LF 2005 Comparative analysis of mitochondrial supercomplexes from summer bamboo Bambusa oldhamii and winter Abstract,The 20th Joint Annual Conference of Biomedical Sciences. Taipei, March, pp.516 .
 
22.Chen MW, Chen PC, Huang CC, Chien LF 2005 Functional expression of mercuric reductase gene in microalga Chlorella DT. Abstract,The 4th Annual Conference of Chinese Phycological Society. Taichung, Jan.
 
23.Chen HP, Chiu, YH, Chien LF 2005 Respiratory characteristic and ascorbate peroxidase activity in bamboo shoot mitochondria. Abstract,The 20th Joint Annual Conference of Biomedical Sciences. Taipei, March, pp.736 .
 
24.Chen M-W, Chien L-F 2004 Functional expression of mercuric reductase (merA) gene in transformed microalga Chlorella DT,Abstract: The 19th Joint Annual Conference of Biomedical Sciences. Taipei, April.