國立中興大學生命科學系

Education

Graduate from	Major in	Degree	Period
University of Cambridge	Department of Biochemistry	PhD	1992 - 1996
National Tsing Hua University	Graduate Institute of Radiation Biology	MSc	1988 - 1990
Chung Yuan Chirstian University	Department of Chemistry	BSc	1982 - 1986

Experience / Honor

Organization	Department	Title	Period
National Chung Hsing University	Department of Life Sciences	Associate Professor	2011.08-now
National Chung Hsing University	Department of Life Sciences	Assistant Professor	2002 - 2011
National Chung Hsing University	Department of Botany	Assistant Professor	2000 - 2002
University of Ben-Gurian (Isreal)	J. Blaustin Insitute of Desert Research	Postdoc	1999 - 2000
National Dong-Hua University	Graduate Institute of Biotechnology	Research Associate	1998 - 1999
Academia Sinica	Insititue of Botany/Molecular Biology	Postdoc	1996 - 1998

Research

Research Interest:
1. Control of energy transduction
2. Photobiological production of green algae

Research Topic :
1. Generation of a zero-CO2-emission factory for photo-biological H2 production in green algal mutants
2. Functional expression of foreign genes in green alga Chlorella.
3. Analysis of energy metablism in bamboo mitochondria: stress

Current Project:
1.Rearch platform of aglae (NCHU101,102,103,104,105)
2.Efficent Solar-to-bioH2: improving solar driven biological H2 production efficiency in green alga Chlorella by genetically engineering (NSC102)
3.Solar-to-bioH2: improving solar driven biological H2 production efficiency in green alga Chlorella by genetically engineering (NSC101)
4.Generation of a zero-CO2-emission green factory for photo-biological H2 production : overexpression of hydrogenase in O2-deprived green algal mutants (NSC99)
5.Proteomic and genomic analysis of bamboo mitochondrial energy-conserving and energy-dissipating systems during shooting stage (NSC96,NSC97)

Lab Member

PhD students: Chou,S-W; Lin, H-D.
Master students: Chen, J-H.
Bachelor students: Chen, Y-Y; Liu, G-L; ..etc.
Graduated students: Chen, H-W (PhD); ..etc

Journal Article

Lin YJ; Chien LF*. 2024/05. Hydrogen production in the Chlorella sp. DT mutants carrying heterologous electron donor ferredoxin 1 of Chlamydomonas reinhardtii., Electronic Journal of Biotechnology [IF=2.3 (2023); Rank=110/174 (63.2%) in BIOTECHNOLOGY & APPLIED MICROBIOLOGY]. vol. 69, pp.11-20.

Tsai HC; Hsieh CH; Hsu CW; Hsu YH; Chien LF.* 2022/04 Cloning and Organelle Expression of Bamboo Mitochondrial Complex I Subunits Nad1, Nad2, Nad4, and Nad5 in the Yeast Saccharomyces cerevisiae.,International Journal of Molecular Sciences [IF=5.924 (2020); Rank=67/295 (22.7%) in BIOCHEMISTRY & MOLECULAR BIOLOGY]. vol.23, pp.4054. (SCI)

Chen TL, Lin YP, Chien CH, Chen YC, Yang YJ, Wang WL, Chien LF, Hsueh HY* 2021/10 Fabrication of Frog-Skin-Inspired Slippery Antibiofouling Coatings Through Degradable Block Copolymer Wrinkling. ,ADVANCED FUNCTIONAL MATERIALS [IF=18.808 (2020), Rank=13/314 (4.1%) in Materials Science-Multidisciplinary]. vol.31, no.42, pp.2170309. (SCI)

Chien LF; Lin WH 2019/09 Changes in photochemical efficiency and differential induction of superoxide dismutase in response to combined stresses of chilling temperature and relatively high irradiation in two Chlorella strains. DOI: 10.5772/intechopen.89024,Intech Open: Microalgae - From Physiology to Application. vol.book chapter. (其他)

Yang, DW; Syn, JW; Hsieh, CH; Huang, CC; Chien, LF* 2019/01 Genetically engineered hydrogenases promote biophotocatalysis-mediated H2 production in the green alga Chlorella sp. DT,International Journal of Hydrogen Energy [IF=4.084 (2018); Rank: 31/103 (30.1%) of Energy & Fuel]. vol.44, no.5, pp.2533-2545. (SCI)

Chen YS, Chesson P, Wu HW, Pao SH, Liu JW, Chien LF, Yong JW, Sheue CR*. 2017/03 Leaf structure affects a plant's appearance: combined multiple-mechanisms intensify remarkable foliar variegation. ,Journal of Plant Research [IF= 1.899 (2016); Rank: 73/212 (36.4%) of PLANT SCIENCES]. vol.130, no.2, pp.311-325. (SCI)

Chen HH, Huang CC, Cheng EH, Lee TH, Chien LF*, Lee MS*. 2017 Optimal timing of blastocyst vitrification after trophectoderm biopsy for preimplantation genetic screening.,PLoS One [IF=2.806 (2016); Rank: 15/128 (11.7%) of MULTIDISCIPLINARY SCIENCES]. vol.12, no.10, pp.e0185747. (SCI)

Hsiu-Hui Chen, Tung-Chuan Yang, Tsung-Hsien Lee, Chun-Chia Huang, Maw-Sheng Lee*, Lee-Feng Chien* 2016/08 Woman age limits the improvable effect of reduced oxygen concentration (5% O2) on human embryo quality and pregnancy potential,International Journal of Clinical and Experimental Medicine.. vol.9, pp.16459-16467. (SCI)

Lin HD, Liu BH, Kuo TT, Tsai HC, Feng TY, Huang CC*, Chien LF*. 2013 Knockdown of PsbO leads to induction of HydA and production of photobiological H2 in the green alga Chlorella sp. DT., Bioresource Technology. [IF= 4.494 (2014); Rank: 13/89 (14.6%) of Energy & Fuels]. vol. 143 , pp.154-162. (SCI)

Chien LF,*, Kuo TT, Liu BH, Lin HD,Feng TY, Huang CC*. 2012/12 Solar-to-bioH2 production enhanced by homologous overexpression of hydrogenase in green alga Chlorella sp. DT.,Internation Journal of Hydrogen Energy (IF=3.313; Rank:25/89 [28.0%]/ Energy & Fuels/ 2014). vol.37, pp.17738-17748. (SCI)

Sheue CR*, Pao SH, Chien LF,Chesson P, Peng CI. 2012 Natural foliar variegation without costs? The case of Begonia,Annals of Botany (London) [IF=3.654 (2014); Rank:26/204 (12.7%) of Plant Science]. vol.109 , pp.1065-1074. (SCI)

Weng JH*, Chien LF, Jiang CY, Shih FC, Chen HY. 2011 A comparison between yellow-green and green cultivars of four vegetable species in pigments, ascorbate, photosynthesis, energy dissipation, and photoinhibition.,Photosynthetica (IF= 1.409; Rank: 99/204 [48.5%] /Plant Sciences / 2014) . vol.49, pp.361-370. (SCI)

Chien LF*, Wu YC, Chen HP. 2011 Mitochondrial energy metabolism in young bamboo rhizomes from Bambusa oldhamii and Phyllostachys edulis during shooting stage.,Plant Physiol. Biochem.(IF=2.756; Rank:44/204 [21.6%]/ Plant Sciences/ 2014). vol.49, pp.449-457. (SCI)

Shieh GJ, Chien LF*, Pan RL 2011 The extrinsic proteins of oxygen-evolving complex in marine diatom Cylindrotheca fusiformis.,Botanical Studies (IF=0.783; Rank:145/204 [71.1%] /Plant Science/ 2014). vol.52, pp.161-171. (SCI)

Chien LF*, Vonshak A. 2011 Enzymatic antioxidant response to low temperature acclimation in the cyanobacterium Arthrospira platensis.,Journal of Applied Phycology (IF=2.559; Rank:18/103 [17.5%], Marine & Freshwater/ 2014). vol.23, pp.887-894. (SCI)

Hsiao Y-Y, Pan Y-J, Hsu S-H, Huang Y-T, Liu T-H, Lee C-H, Lee C-H, Liu P-F, Chang W-C, Wang Y-K, Chien L-F, Pan R-L* 2007 Functional roles of arginine residues in mung bean vacuolar H+-pyrophosphatase. ,Biochim. Biophys. Acta -Bioenergetics (IF=5.353; Rank: 11/74 [14.9%] / Biophysics/ 2014) . vol.1767, pp.965-973. (SCI)

Huang C-C, Chen M-W, Hsieh J-L, Lin W-H, Chen P-C, Chien L-F*. 2006 Expression of mercuric reductase from Bacillus megaterium MB1 in eukaryotic microalga Chlorella sp DT,Appl. Microb. Biotechnol. (IF=3.337; Rank: 39/163 [23.9%]/ Biotechnology & Applied Microbiology/ 2014). vol.72, pp.197-205. (SCI)

Lee SC, Chien LF*, Van RC, Hsiao YY, Hong JL, Pan RL. 2006 Radiation inactivation analysis of thylakoid protein kinase systems in light and in darkness,Photosynthetica. (IF= 1.409; Rank: 99/204 [48.5%] / Plant Sciences / 2014). vol.44, pp.116-124. (SCI)

Kuo SY, Chien LF, Van RC, Yan KH, Liu PF, Chang WC, Wang JK, Pan RL 2005 Purification and subunit determination of inorganic H+-pyrophosphatase from endoplasmic reticulum-enriched vesicles of mung bean seedlings,Plant Sci. 169. pp.847-853. (SCI)

Kuo SY, Chien LF, Hsiao YY, Van RC, Yan KH, Liu PF, Mao SJ, Pan RL 2005 Proton pumping inorganic pyrophosphatase of endoplasmic reticulum-enriched vesicles from etiolated mmung bean seedlings,J. Plant Physiol. 162. pp.129-138. (SCI)

Symposium Presentation

Kuo TT, Lin YJ, ChienLF*. 2017/09 Invited speaker: Green and clean bioenergy H2 produced by green alga Chlorella sp. DT transgenics during biophotolysis. Abstract: 3rd World Congress and Expo on Green Energy. 28-29 September 2017, Berlin, Germany,3rd World Congress and Expo on Green Energy. 28-29 September 2017, Berlin, Germany. (其他)

Ting-Ting Kuo, Yen-Ju Lin, Lee-Feng Chien* 2016/08 Invited speaker: Clean bioenergy H2 photobiologically produced by green algal Chlorella sp. DT mutants with overexpressed hydrogenase or ferredoxin.,6th International Symposium on Energy Challenges and Mechanics. Towards a Big Picture.. Inverness, Scotland, UK. (其他)

Ting-Ting Kuo, Yen-Ju Lin, Lee-Feng Chien* 2016 Invited speaker: Clean energy H2 photobiologically produced by green algal Chlorella sp. DT mutants with overexpressed hydrogenase or ferredoxin. Abstract.,2016光合作用與植物生理研討會. Taipei, Taiwan. (其他)

Syu JW, Yang DW, Chien LF. 2014 The hydrogen production of high oxygen tolerance hydrogenase mutant of Cholrella sp. DT. Poster Abstract: 2014 Plant Science Annual Meeting & Symposium,13-14 December 2014, Taipei, Taiwan.

Lin YJ, Yang DW, Chien LF 2014 Construction of p121-crfd-gfp vector for overexpressing ferredoxin 1 in Chlorella sp. DT hydrogenase mutants. Poster Abstract:2014 Plant Science Annual Meeting & Symposium,13-14 December 2014,Taipei, Taiwan..

Kuo TT, Liu BH, Lin HD, Feng TY, Huang CC, Chien LF. 2014 Overexpression of HydA or knockdown of PsbO lead to the increase in the photobiological H2 production in green alga Chlorella sp. DT. Poster Abstract: The 15th IUBMB-24th FAOBMB-TSBMB Conference. 21-26th Oct, 2014, Taipei, Taiwan.

Kuo TT, Liu BH, Lin HD, Feng TY, Huang CC, Chien LF*. 2014 Kuo TT, Liu BH, Lin HD, Feng TY, Huang CC, Chien LF. (2014) Overexpression of HydA or knockdown of PsbO lead to the increase in the photobiological H2 production in green alga Chlorella sp. DT. Poster Abstract. ,The 15th IUBMB-24th FAOBMB-TSBMB Conference. 21-26 October 2014, Taipei, Taiwan. (其他)

Hsieh CW, Chen LF. 2013/03 Expression of mitochondrial-encoded Nad2 and Nad5 subunits of bamboo Phyllostachys edulis mitochondrial NADH:ubiquinone oxidoreductase in Saccharomyces cerevisiae. Poster Abstract: The 28th Joint Annual Conference of Biomedi,The 28th Joint Annual Conference of Biomedi. Taipei, Taiwan. (其他)

Hsu CW, Chen LF 2013 Expression of mitochondrial-encoded Nad2 and Nad5 subunits of bamboo Phyllostachys edulis mitochondrial NADH:ubiquinone oxidoreductase in Saccharomyces cerevisiae. Poster Abstract:,The 28th Joint Annual Conference of Biomedical Sciences, 16-17 March, 2013. Taipei, Taiwan.

Yang DW, Chen LF 2013 Green alga Chlorella hydrogenase mutants that have high O2 tolerance. Poster Abstract: The 28th Joint Annual Conference of Biomedical Sciences,16-17 March, 2013, Taipei, Taiwan. P726.

Lin HD, Liu BH, Kuo TT, Chien LF*. 2012 Invited speaker: (IFIB-2012 Biorefinery Technology 7) Knockdown of PsbO leads to the induction of HydA and the production of photobiological H2 in green alga Chlorella sp. DT, Abstract: IFIB-2012 5th International Conference on Industrial Bioprocess in Taiwan 7-10 October, 2012.

Hsieh CH, Chein LF 2011 Expression of bamboo mitochondrial-encoded Nad1 subunit in Saccharomyces cerevisiae,The 26th Joint Annual Conference of Biomedical Sciences. Taipei, March, 2011. #P1. .

Chien LF, Lin CD, Kou TT 2010 Production of H2 by solar-driven pathway as electrons from photosynthetic electron transport chain being transferring to hydrogenase,Satellite Meeting of the 15th International Photosynthesis Congress, ISPR, Singapore, August, . pp.38.

Liu BH, Chien LF 2010 Construction of the RNA interference Vectors to knockdown PsbO protein in Chlorella sp DT,The 25th Joint Annual Conference of Biomedical Sciences. Taipei, March, pp.434.

Kou TT, Chien LF 2010 Homologous overexpression of hydrogenase in Chlorella sp. DT to enhance hydrogen production,The 25th Joint Annual Conference of Biomedical Sciences. Taipei, March, 2010, pp.449.

Lin HD, Chien LF 2009 Using RNA interference to generate D1-deficient or PsbO-deficient Chlorella sp DT for hydrogen production,The 24th Joint Annual Conference of Biomedical Sciences. Taipei, March, , pp.735.

Chien LF, Wu YC, Ko GC 2009 Mitochondrial proteome in young bamboo rhizome from Bambusa oldhamii and Phyllostachys edulis,Abstract: Plant Biology . Hawaii, July, pp.45021.

Chiu CY, Chien LF 2009 Proteomic analysis of merA transgenic Chlorella by IEF/SDS-PAGE,The 24th Joint Annual Conference of Biomedical Sciences. Taipei, March, pp.740.

Wang SH, Chien LF 2009 Expression and characterization of bamboo mitochondrial-encoded Cox2 subunit in yeast,The 24th Joint Annual Conference of Biomedical Sciences. Taipei, March, pp.736.

Chien LF, Chen HP, Wu YC 2008 Bamboo mitochondrial energy metabolic pathways in Bambusa oldhamii and PhyllostBiochim achys edulis during rapid shooting stage,Acta-Bioenergetics 1777: S98. #S13.41. 15th European Bioenergetics Conference, Dublin, Ireland, 19-24th July 2008.

Tsai HC, Hsu YH, Chien LF 2007 Genomic analysis of plant mitochondrial nd1 and nd5 subunits of nadh:ubiquinone oxidoreductase,The 22nd Joint Annual Conference of Biomedical Sciences. Taipei, March, pp.355.

Huang HS, Chang HY, Chen HP, Lau YY, Wu GW, Shi SH, Tsai HC, Chien LF 2007 Variation in photosynthesis, respiration, and superoxide dismutase activity in Bolboschoenus planicu ,The 22nd Joint Annual Conference of Biomedical Sciences. Taipei, March, pp.988.

Chien LF, Chen MW, Huang CC 2006 Mercury phytoremediation: functional expression of mercuric reductase from Bacillus megaterium MB1 i Abstract:Plant Biology 2006,5-9 August, Boston. pp.06024.