顏宏真 - 教授
顏宏真 教授 繁體中文 English
專任教師
生理
04-22840416#520、521

高等植物耐鹽性的生理生化與分子機制

學歷

畢業學校 主修學門系所 學位 時期
美國華盛頓州立大學 植物生理 博士 1989-1993
美國加州大學河濱分校 植物系 碩士 1987-1989
國立台灣大學 植物系 學士 1983-1987

經歷 / 榮譽

經歷

服務機關 服務部門 職稱 時期
國立中興大學 生命科學系 教授 2002~迄今
University of Illinois, Champaign Carl R. Woese Institute for Genomic Biology Visiting scholar 2018/03-2018/06
Washington State University Biological Science VisitingScholar 2006/02~2006/07
Kobe University Biological Science Visitingscholar 2013/04-2013/08
國立中興大學 植物系 副教授 1995~2002

榮譽

年份

標題

2009

97年度優良導師

2014

102年度教學特優

研究方向

鹽害及乾旱是限制植物分佈和產量重要的因素,由於人類耕作大量施予無機肥,土壤中累積的鹽分漸漸提高造成鹽害,尤其以鈉鹽的累積影響最大。據估計全球三分之一以上的灌溉土地均有鹽害的問題存在,大部份的作物對鹽分均相當敏感,因此,如何減緩鹽分對作物的影響,使作物的耐鹽程度提高,是農業發展的重要課題之一。耐鹽植物能生長在含有較高的鹽分的土壤中,表示此類植物對鹽分的忍受能力較高,而高等植物的耐鹽機制是多途徑的,研究其耐鹽的相關機制,再應用至作物上,對提高作物對鹽份的耐受性應有助益。

目前本實驗室以耐鹽植物冰花為模式,進行一系列高等植物耐鹽性之分子機制研究。耐鹽植物冰花(Mesembryanthemum crystallinum; ice plant)是雙子葉一年生植物,幼苗時並不耐鹽,如遭遇乾旱、高鹽環境或發育至成熟階段時,會發生許多基因層次的變化,造成許多生理生化途徑之變化,以防止體內水分散失及離子毒害,適應環境的變化。冰花可生長於高鹽分(500 mM NaCl)的土壤中,且其耐鹽性可經由環境變化而誘導,並具有細胞層次的耐鹽性,是研究高等植物耐鹽機制的模式植物。目前研究高等植物的耐鹽機制有幾個方向:水分吸收,相容性溶質的累積,鈉離子區隔,以及鉀離子的平衡。近期研究乃針對鹽逆境下鉀離子的吸收做深入探討,但由於冰花不適合進行遺傳操作,故利用酵母菌及阿拉伯芥完備之遺傳系統同步進行相關基因功能之研究。

期刊論文

 
Li CH, Tu YC, Wen MF, Tien HJ, Yen HE* (2023) Exogenous myo-inositol increases salt tolerance and accelerates CAM induction in the early juvenile stage of the facultative halophyte Mesembryanthemum crystallinum but not in the late juvenile stage. Functional Plant Biology 50: 363-377 (SCI)
Li CH, Tien HJ, Wen MF, Yen HE* (2021) Myo-inositol transport and metabolism participate in salt tolerance of halophyte ice plant seedlings. Physiologia Plantarum 172: 1619-1629. (SCI)
Hwang HH, Wang CH, Huang HW, Chiang CP, Chi SF, Huang FC, Yen HE* (2019) Functional analysis of McSnRK1 (SNF1‑related protein kinase 1) in regulating Na/K homeostasis in transgenic cultured cells and roots. Plant Cell Reports 38: 915-926. (SCI)
Hwang HH, Wang CH, Chen HH, Ho JF, Chi SF, Huang FC, Yen HE (2018) Effective Agrobacterium-mediated transformation protocols for callus and roots of halophyte ice plant (Mesembryanthemum crystallinum),Botanical Studies 60: 1-15. (SCI)
Chiang C-P, Yim WC, Sun Y-H, Ohnishi M, Mimura T, Cushman JC, Yen HE 2016 Identification of ice plant (Mesembryanthemum crystallinum L.) microRNAs using RNA-Seq and their putative roles in high salinity responses in seedlings. Frontiers in Plant Science. 7: 1143. (SCI)
Lin YC, Yen HE (2015) The effects of salt stress on the growth and the patterns of SUMO-conjugated polypeptides in Arabidopsis SUMO loss-of-function mutants,Journal of Central Taiwan University of Science and Technology 25: 49-72.
Li CH, Chiang CP, Yang JY, Ma CJ, Chen YC, Yen HE* (2014) RING-type ubiquitin ligase McCPN1 catalyzes UBC8-dependent protein ubiquitination and interacts with Argonaute 4 in halophyte ice plant. Plant Physiology and Biochemistry 80: 211-219. (SCI)
Lai SJ, Lai MC, Lee RJ, Chen YH, Yen HE (2014) Transgenic Arabidopsis expressing osmolyte glycine betaine synthesizing enzymes from halophilic methanogen promote tolerance to drought and salt stress. Plant Molecular Biology 85: 429-411. (SCI)
Liu CW, Chang TS, Hsu YK, Wang AZ, Yen HC, Wu YP, Wang CS, Lai CC (2014) Comparative proteomic analysis of early salt stress responsive proteins in roots and leaves of rice. Proteomics 14: 1759-1775. (SCI)
Chiang CP, Li CH, Jou Y, Chen YC, Lin YC, Yang FY, Huang NC, Yen HE (2013) Suppressor of K+ Transport Growth Defect 1 (SKD1) interacts with RING-type ubiquitin ligase and sucrose non-fermenting 1-related protein kinase (SnRK1) in halophyte ice plant. J Exp Bot. 64: 2385–2400. (SCI)
Jou Y, Chiang CP, Yen HE (2013) Changes in cellular distribution regulate SKD1 ATPase activity in response to a sudden increase in environmental salinity in halophyte ice plant. Plant Signaling & Behavior 8:12.
Liu CW, Hsu YK, Cheng YH, Yen HC, Wu YP, Wang CS, Lai CC (2012) Proteomic analysis of salt-responsive ubiquitin-related proteins in rice roots. Rapid Commun Mass Spectrom. 26: 1649-1660. (SCI)
Ho L-W, Yang T-T, Shieh S-S, Edwards GE, Yen HE* (2010) Reduced expression of a vesicle trafficking-related ATPase SKD1 decreases salt tolerance in Arabidopsis,Functional Plant Biology 37: 962-973. (SCI)
Huang NC, Li CH, Lee JY, Yen HE* (2010) Cytosine methylation changes in the ice plant Ppc1 promoter during transition from C3 to Crassulacean acid metabolism. Plant Science 178: 41-46. (SCI)
Jwo WS, Chern CG, Lai MH, Yen HM, Tseng TH, Yen HE (2008) Screening technique for salt tolerance in the rice seedling stage. J Taiwan Agric Res 57: 193-204.
Ho TF, Ma CJ, Lu CH, Tsai YT, Wei YH, Chang JS, Lai JK, Cheuh PJ, Yeh CT, Tang PC, Tsai Chang J, Ko JL, Liu FS, Yen HE, Chang CC. (2007) Undecylprodigiosin selectively induces apoptosis in human breast carcinoma cells independent of p53. Toxicology and Applied Pharmacology 225: 318–328. (SCI)
Jou Y, Wang YL, Yen HE* (2007) Vacuolar acidity, protein profile, and crystal composition of epidermal bladder cells of the halophyte Mesembryanthemum crystallinum. Functional Plant Biology 34: 353-359. (SCI)
Jou Y, Chiang CP, Jauh GY, Yen HE* (2006) Functional characterization of ice plant SKD1, an AAA-type ATPase associated with ER-Golgi network, and its role in adaptation to salt stress. Plant Physiology 141: 135-146. (SCI)
Jou Y, Chou PH, He M, Hung Y, Yen HE* (2004) Tissue-specific expression and functional complementation of a yeast potassium-uptake mutant by a salt-induced ice plant gene mcSKD1. Plant Molecular Biology 54: 881-893. (SCI)
Yang J, Yen HE* (2002) Early salt stress effects on the changes in chemical composition in leaves of Mesembryanthemum crystallinum. Plant Physiology 130: 1032-1042. (SCI)
Yen SK, Chung MC, Chen PC, Yen HE* (2001) Environmental and developmental regulation of a wound-induced cell wall protein WI12 in halophyte Mesembryanthemum crystallinum. Plant Physiology 127. 517-528. (SCI)
Yen HE*, Wu SM, Hong YH, Yen SK (2000) Isolation of 3 salt-induced low-abundance cDNAs from light-grown callus of Mesembryanthemum crystallinum. Physiologia Plantarum 110: 402-409. (SCI)
Yen HE*, Chen YC, Yen SK, Lin JH (1999) Sugar uptake by photomixotrophic soybean suspension cultures. Bot. Bull. Acad. Sinica 40: 147-152. (SCI)
Yen SK, Chen PC, Yen HE* (1999) Cloning of a wound-induced gene WI12 from Mesembryanthemum crystallinum (Accession No. AF117224). Plant Physiology 119: 1147. (SCI)
Yen SK, Chen PC, Yen HE* (1999) Characterization of a gene encoding ubiquitin-conjugating enzyme from Mesembryanthemum crystallinum. Plant Physiology 121: 538. (SCI)
Yen HE* and Yen SK (1999) Effect of high salinity on trachery element differentiation in light-grown callus of Mesembryanthemum crystallinum. Plant Cell Tissue & Organ Culture 58: 59-65. (SCI)
Yen HE*, Zhang D, Lin JH, Edwards GE, Ku MSB (1997) Salt-induced changes in protein composition in light-grown callus of M. crystallinum. Physiologia Plantarum 101: 526-532. (SCI)
Yen HE, Grimes HD, Edwards GE* (1995) The effects of high salinity, water-deficit, and abscisic acid on phosphoenolpyruvate carboxylase activity and proline accumulation in Mesembryanthemum crystallinum cell cultures. J. Plant Physiol. 145: 557-564. (SCI)
Yen HE, Edwards GE and Grimes HD* (1994) Characterization of a salt-responsive 24-kilodalton glycoprotein in M. crystallinum. Plant Physiology 105: 1179-1187. (SCI)

研討會論文

 
Y Jou, S-S Shieh, H.E. Yen. 2001 Potassium effects on salt-stressed suspension cells of halophyte Mesembryanthemum crystallinum and g International symposium on plant environment interactions,genes, proteins, and biotechnology, abstract 17. pp.34.
S-K Yen and H.E. Yen. 2000 Analyses of transcript and protein accumulation of a wound-induced protein WI12 in ice plant Mesembr Annual Meeting of American Society of Plant Physiologists,San Diego, Abs 686.
H.E. Yen, S-M Wu, Y-H Hong, J-H Lin. 2000 The cellular mechanisms of salt tolerance in higher plants,2000植物分子生物學新知暨生物技術產業化研習會.
S-K Yen and H.E. Yen. 1998 Characterization of a wound-induced gene in ice plant. ,中華民國植物學年會第23屆大會.
Y-H Hong, S-M Wu and H.E. Yen. 1998 Sequence and expression analysis of a salt-induced SKD 1 gene in halophyte ice plant,中華民國植物學年會第23屆大會.
S-K Yen and H.E. Yen. 1997 Cloning of an wound-induced PR-like protein from light-grown callus of common ice plant,The 5th International Congress of Plant Molecular Biology. Singapore. Abs 679.

研究計劃

年度
補助機關
計畫名稱
計畫起迄日期
2022
國家科學及技術委員會
利用阿拉伯芥int突變株來鑑定耐鹽植物冰花INT家族之功能
2022/08/012023/07/31
2021
國家科學及技術委員會
冰花肌醇運輸蛋白家族參與耐鹽性功能分析
2021/08/012022/07/31
2020
科技部
肌醇運輸與代謝參與冰花耐鹽機制之探討
2020/08/012021/07/31
2019
科技部
以RNA-seq分析耐鹽植物冰花miRNA參與後轉錄調控高鹽逆境的初始反應
2019/08/012020/07/31
2018
科技部
以RNA-seq分析耐鹽植物冰花miRNA參與後轉錄調控高鹽逆境的初始反應
2018/08/012019/07/31
2017
科技部
以RNA-seq分析耐鹽植物冰花miRNA參與後轉錄調控高鹽逆境的初始反應
2017/08/012018/07/31
2016
科技部
新興高經濟價值蔬菜冰花抗氧化相關酵素活性及基因表現分析
2016/08/012017/07/31
2015
科技部
新興高經濟價值蔬菜冰花抗氧化相關酵素活性及基因表現分析
2015/08/012016/07/31
2014
科技部
建立冰花培養細胞與根部的穩定轉殖系統以進行耐逆境基因SnRK1功能分析
2014/08/012015/07/31
2013
行政院國家科學委員會
建立大量表現高鹽太古生物甜菜鹼生合成基因之耐鹽植物轉殖系
2013/08/012014/10/31
2012
行政院國家科學委員會
建立大量表現高鹽太古生物甜菜鹼生合成基因之耐鹽植物轉殖系
2012/08/012013/07/31
2011
行政院國家科學委員會
E3 ubiquitin ligase mcCPN1參與耐鹽植物冰花鈉鉀離子平衡機制
2011/08/012012/07/31
2011
行政院國家科學委員會
建立大量表現高鹽太古生物甜菜鹼生合成基因之耐鹽植物轉殖系
2011/08/012012/07/31
2010
行政院農業委員會農糧署
作物基因轉移技術之開發及應用計畫
2010/01/012010/12/31
2010
行政院國家科學委員會
E3 ubiquitin ligase mcCPN1參與耐鹽植物冰花鈉鉀離子平衡機制
2010/08/012011/07/31
2009
行政院國家科學委員會
E3 ubiquitin ligase mcCPN1參與耐鹽植物冰花鈉鉀離子平衡機制
2009/08/012010/07/31
2009
行政院農業委員會農糧署
作物基因轉移技術之開發應用-利用基因轉殖技術開發黃色或藍紫色系列之蝶蝶蘭品種
2009/02/272009/12/31
2008
行政院國家科學委員會
耐鹽相關蛋白SKD3參與蛋白運輸及訊息傳遞之過程
2008/08/012009/07/31
2008
行政院農業委員會農糧署
作物基因轉移技術之開發應用-花卉生技關鍵技術之開發─利用基因轉殖技術育成黃色或藍紫色系列之蝴蝶蘭品種
2008/04/082008/12/31
2008
行政院農業委員會
分子生物產業應用及技術平台之建立-香味水稻之功能基因體學研究子計畫3:香味成分生成與移轉之生理機制探討
2008/01/012008/12/31
2007
行政院國家科學委員會
耐鹽相關蛋白SKD2參與蛋白運輸及訊息傳遞之過程
2007/08/012008/07/31
2007
行政院農業委員會
分子生物產業應用及技術平臺之建立─香味水稻之功能基因體學研究子計畫三─香味成分生成與移轉之生理機制探討
2007/01/012007/12/31
2006
行政院國家科學委員會
耐鹽相關蛋白SKD1參與蛋白運輸及訊息傳遞之過程
2006/08/012007/07/31
2005
行政院國家科學委員會
香味水稻之功能基因體學研究
2005/08/012006/12/31
2005
行政院國家科學委員會
鹽逆境誘導耐鹽植物冰花蛋白質組成和次級結構之變化(3/3)
2005/08/012006/07/31
2004
行政院國家科學委員會
鹽逆境誘導耐鹽植物冰花蛋白質組成和次級結構之變化(2/3)
2004/08/012005/07/31
2003
行政院國家科學委員會
鹽逆境誘導耐鹽植物冰花蛋白質組成和次級結構之變化(1/3)
2003/08/012004/07/31
2003
行政院國家科學委員會
高效率之鉀離子運輸與細胞耐鹽性之分子機制探討(3/3)
2003/08/012004/07/31
2002
行政院國家科學委員會
高效率之鉀離子運輸與細胞耐鹽性之分子機制探討(2/3)
2002/08/012003/07/31
2001
行政院國家科學委員會
高效率之鉀離子運輸與細胞耐鹽性隻分子機制探討(1/3)
2001/08/012002/07/31