王隆祺 - 副教授
王隆祺 副教授 繁體中文 English
專任教師
生理
04-22840416#617、618

物荷爾蒙生理調控與分子遺傳機制

學歷

畢業學校 主修學門系所 學位 時期
美國亞伯愛因斯坦醫學院 細胞生物學系 博士 1992~1998
國立中山大學 海洋資源系 學士 1983~1987

經歷 / 榮譽

服務機關 服務部門 職稱 時期
國立中興大學 生命科學系 助理教授 2015~迄今
國立中興大學 生物科技發展中心 助研究員 2013~2015
中央研究院 植物暨微生物學研究所 助研究員 2004~2013
沙克研究所 植物學研究所 博士後研究員 2000~2004
賓州大學 生物學系 博士後研究員 1998~2000
中央研究院 分子生物學研究所 研究助理 1989~1991

研究方向

以植物荷爾蒙乙烯為研究主題,探討乙烯與其他植物荷爾蒙之交互作用及其調控植物生理與發育之分子機制,進行基礎學理研究及開發相關農業生物技術。由於乙烯可調節果實後熟及花卉壽命,我們的研究目標與預期成果具有實際的農業應用價值。
我們利用模式植物阿拉伯芥 (Arabidopsis thaliana),以植物生理學、生化學、分子生物學與遺傳學的方法,研究乙烯之生理功能及其分子調控機制。承續先前的研究成果,我們利用化學遺傳學 (Chemical Genetics) 的方法,篩選得到一些新穎的小分子化合物,具有潛力作為調控乙烯生理功能的農用化合物,部分化合物業已申請得到台灣及美國的新型發明專利。目前研究室利用阿拉伯芥及酵母菌 (Saccharomyces cerevisiae) 雙雜合系統 (Yeast Two-hybrid System) 進行開發更為專一之小分子化合物,用以調控乙烯生合成及生理功能,期能應用於高經濟價值之切花,例如文心蘭,降低採收後儲運的損失並能延長瓶插壽命,而提高市場價值及增加台灣出口花卉產業的國際競爭力。此外,篩選所得之小分子化合物,也可以藉由調控乙烯的訊息傳遞途徑 (Signaling transduction pathway) ,而增加植物對生物與非生物逆境的耐受性及適應能力。

研究室人員

碩士班(二年級)
謝睿旂
蘇耀庭

碩班畢業生
林詩霖 (黃皓瑄老師共同指導)
李賢啟
蕭筑尹
許景淯
呂宜螢
許皓婷
蘇培欽
田詠欣

博班畢業生
托路可

實驗室前成員
劉恭明 (博士後研究員)
謝文儒 (兼任研究助理)
游瑞誠 (專任研究助理)

**** 歡迎對植物科學有興趣的研究生加入我們植物荷爾蒙的研究團隊 ****

期刊論文

 
Islam MT, Wang LC*, Chen IJ, Lo KL, Lo WS 2021/08 Arabidopsis JMJ17 promotes cotyledon greening during de-etiolationby repressing genes involved in tetrapyrrole biosynthesis in etiolated seedlings.,New Phytologist. DOI: https://doi.org/10.1111/nph.17327. . vol.231, pp.1023-1039. (SCI)
Gu, S.Y., Lo, W.S., Wu, S.J., and Wang LC* 2021/03 Dimerization of the ETO1 family proteins plays a crucial role in regulating ethylene biosynthesis in Arabidopsis thaliana.,Plant Journal. DOI: https://doi.org/10.1111/tpj.15111. vol.105, pp.1293-1308. (SCI)
Gu SY#, Wang LC*, Cheuh CM, Lo WS* 2019/05 CHITINASE LIKE1 regulates root development of dark-grown seedlings by modulating ethylene biosynthesis in Arabidopsis thaliana.,Frontiers in Plant Science. DOI: https://doi.org/10.3389/fpls.2019.00600. vol.10, pp.600. (SCI)
Catinot, J., Huang, J.B., Huang, P.Y., Tseng, M.Y., Chen, Y.L., Gu, S.Y., Lo, W.S., Wang, L.C., Chen, Y.R., and Zimmerli, L. 2015/12 ETHYLENE RESPONSE FACTOR 96 positively regulates Arabidopsis resistance to necrotrophic pathogens by direct binding to GCC elements of jasmonate- and ethylene-responsive defence genes.,Plant, Cell & Environment. DOI: https://doi.org/10.1111/pce.12583. vol.38, pp.2721-2734. (SCI)
Wang, L.-C., Montalvo-Munoz, F., Tsai, Y.-C., Liang, C.-Y., Chang, C.-C., and Lo, W.-S. 2015 The histone acetyltransferase Gcn5 regulates ncRNA-ICR1 and FLO11 Expression during pseudohyphal development in Saccharomyces cerevisiae.,BioMed Research International, vol. 2015, Article ID 284692. doi:10.1155/2015/28469.
Lin, L.-C., Chueh, C.-M., and Wang, L.-C. 2014 Investigating the phytohormone ethylene response pathway by chemical genetics in Plant Chemical Genomics: Methods in Molecular Biology,vol. 1056: 63-77 (Humana Press), (book chapter).. (SCI)
Lo, K.-L.§, Wang, L.-C.§, Chen, I-J., Liu, Y.-C., Chung, M.-C., and Lo, W.-S. 2014 Transcriptional consequence and impaired gametogenesis with high-grade aneuploidy in Arabidopsis thaliana,PLoS ONE. vol.9, no.12. (SCI)
Liang, C.-Y., Wang, L.-C., and Lo, W.-S. 2013 Dissociation of the H3K36 demethylase Rph1 from chromatin mediates derepression of environmental stress-response genes under genotoxic stress in Saccharomyces cerevisiae.,Molecular Biology of the Cell. vol.24(20), pp.3251-326. (SCI)
Chen, I-J., Lo, W.-S., Chuang, J.-Y., Chueh, C.-M., Fan, Y.-S., Lin, L.-C., Wu, S.-J., and Wang, L.-C. 2013 Chemical genetics reveals a role of brassinolides and cellulose synthase in the hypocotyl development of etiolated Arabidopsis seedlings.,Plant Science, 209: 46-5. (SCI)
Chen, G.-H., Chan, Y.-L., Liu, C.-P., and Wang, L.-C. 2012 Ethylene response pathway is essential for ARABIDOPSIS A-FIFTEEN function in floral induction and leaf senescence.,Plant Signaling & Behavior, 7(4): 457-46.
Chen, G.-H., Liu, C.-P., Chen S.-C., and Wang, L.-C. 2012 Role of ARABIDOPSIS A-FIFTEEN in regulating leaf senescence involves response to reactive oxygen species and is dependent on ETHYLENE INSENSITIVE2.,Journal of Experimental Botany, 63(1): 275-29. (SCI)
Liang, C.-Y., Hsu, P.-H., Chou, D.-F., Pan, C.-Y., Wang, L.-C., Huang, W.-C., Tsai M.-D. and Lo, W.-S. 2011 The histone H3K36 demethylase Rph1/KDM4 regulates the expression of the photoreactivation gene PHR1.,Nucleic Acids Research, 39(10): 4151-416. (SCI)
Lin, L.-C., Hsu, J.-H. and Wang, L.-C. 2010 Identification of novel inhibitors of 1-aminocyclopropane-1-carboxylic acid synthase by chemical screening in Arabidopsis thaliana.,Journal of Biological Chemistry, 285(43): 33445-3345. (SCI)
Chilley, P.M., Casson, S.A., Tarkowski, P., Wang, K. L.-C., Hawkins, N., Hussey, P.J., Beale, M., Ecker, J.R., Sandberg, G.K., and Lindsey, K. 2006 The POLARIS peptide of Arabidopsis regulates auxin transport and root growth via effects on ethylene signaling.,Plant Cell, 18(11): 3058-307. (SCI)
Yoshida, H.*, Wang, K. L.-C., Chang, C.-M., Mori, K., Uchida, E., and Ecker, J.R. 2006 The ACC synthase TOE sequence is required for interaction with ETO1 family proteins and destabilization of target proteins.,Plant Molecular Biology. vol.62, pp.427-43. (SCI)
Yoshida, H.*, Nagata, M., Saito, K., Wang, K. L.-C., and Ecker, J.R. 2005 Arabidopsis ETO1 specifically interacts with and negatively regulates type 2 1-aminocyclopropane-1-carboxylate synthases.,MBC Plant Biology. vol.5, pp.1. (SCI)
Wang, K. L.-C.§, Yoshida, H.§., Lurin, C.§, and Ecker, J.R. 2004 Regulation of ethylene gas biosynthesis by Arabidopsis ETO1 protein,Nature. vol.428, pp.945-950. (SCI)
Wang, K. L.-C., Li, H., and Ecker, J.R. 2002 Ethylene biosynthesis and signaling networks,Plant Cell. vol.14. (SCI)
Wang, K. L.-C. and Warner, J.R 1998 Postive and negative autoregulation of REB1 transcription in Saccharomyces cerevisiae,Molecular and Cellular Biology. vol.18, pp.4368-437. (SCI)

研討會論文

 
Islam MT*, Wang LC, Chen IJ, Lo KL and Lo WS 2020/07 JMJ17 promotes cotyledon greening during de-etiolation through repressing genes involved in tetrapyrrole biosynthesis in etiolated Arabidopsis seedlings.,Plant Biology Worldwide Summit. Washington, DC, USA..
Jui-Cheng Yu, Kung-Ming Liu, Sian-Chi Li, Wan-Sheng Lo, and Long-Chi Wang 2019/11 Chemical interferece of ETHYLENE INSENEITIVE3 dimerization by small-molecule compounds to modulate ethylene response in Arabidopsis thaliana,2019 TSPB Annual Meeting and Symposium. Academia Sinica, Taipei, Taiwan.
Ching-Yu Hsu, Shin-Yuan Gu, Wan-Sheng Lo, and Long-Chi Wang 2019/11 A potential role of Arabidopsis ETHYLENE OVERPORDUCER1 in the nucleus,2019 TSPB Annual Meeting and Symposium. Academia Sinica, Taipei, Taiwan.
Shin-Yuan Gu, Shaw-Jye Wu, Long-Chi Wang, and Wan-Sheng Lo 2019/11 Dimerization of the BTB protein, ETO1 and EOLs, plays a crucial role in regulaing ethylene biosynthesis in Arabidopsis thaliana,2019 TSPB Annual Meeting and Symposium. Academia Sinica, Taipei, Taiwan.
Islam MT, Wang LC, Chen IJ, Lo KL, and Lo WS 2019/10 AtJMJ17, a H3K4 demethylase promotes seedling greening through repressing tetrapyrrole biosynthesis genes in the dark.,The 14th Asia Epigenome Meeting (AEM) and 3rd Taipei Epigenetics and Chromatin Meeting (TECM).. Academia Sinica, Taipei, Taiwan..
Lin WH, Wang LC, Huang SC, and Lo WS 2019/10 H3K36 demethylase Rph1 modulates the transcriptional reprogramming of cellular quiescence during chronological aging in Saccharomyces cerevisiae.,The 14th Asia Epigenome Meeting (AEM) and 3rd Taipei Epigenetics and Chromatin Meeting (TECM).. Academia Sinica, Taipei, Taiwan..
Lin WH, Wang LC, Huang SC, and Lo WS 2019/08 H3K36 demethylase Rph1 modulates the transcriptional reprogramming of cellular quiescence during chronological aging in Saccharomyces cerevisiae.,Meeting report from Taiwan Yeast Meeting.. Academia Sinica, Taipei, Taiwan..
Islam MT, Wang LC, Chen IJ, Lo KL, and Lo WS 2019/03 Light-mediated seedling development in Arabidopsis thaliana regulated by AtJP1, a H3K4 demethylase.,Meeting report from Japan-Taiwan Plant Biology International Conference (日台植物生物學國際研討會). Nagoya University, Japan..
Jui-Cheng Yu, Kung-Ming Liu, Sian-Chi Li, Wan-Sheng Lo, and Long-Chi Wang 2019/03 Chemical interferece of ETHYLENE INSENEITIVE3 dimerization by small-molecule compounds to modulate ethylene response in Arabidopsis thaliana,Japan-Taiwna plant biology 2019. Nagoya University, Nagoya, Japan.
Ching-Yu Hsu, Yi-Ying Lu, Hao-Ting Hsu, Wan-Sheng Lo, and Long-Chi Wang 2019/03 A potential role of Arabidopsis ETHYLENE OVERPRODUCER1 in nucleus,Japan-Taiwan Plant Biology 2019. Nagoyu University, Nagoya, Japan.
Lin WH, Wang LC, Huang SC, and Lo WS 2018/08 H3K36 demethylase Rph1 modulates the transcriptional reprogramming of cellular quiescence during chronological aging in Saccharomyces cerevisiae.,Taiwan Yeast Meeting.. National Taiwan University, Taipei, Taiwan..
Kung-Ming Liu, Jui-Cheng Yu, Sian-Chi Li, Jhu-Yin Siao, Wan-Sheng Lo, and Long-Chi Wang 2018/08 Idenrification and characterization of small-molecule chemical inhibitors of Arabidopsis EIN3 function,2018植物分子生物夏令營暨植物學年會(Taiwan Society of Plant Biologists Summer Workshop and Annual Meeting. Tainan走馬瀨農場.
Hao-Ting Hsu, Wen-Ju Hsieh, Kung-Ming Liu, Wang-Sheng Lo, and Long-Chi Wang 2018/08 Chemical interrogation of the functional triad, ETR1-CTR1-EIN2, in ethylenesignal transduction pathway in Arabidopsis thaliana,2018植物分子夏令營暨植物學年會(Taiwan Society of Plant Biologists Summer Workshop and Annual Meeting). Tainan走馬瀨農場.
Yi-Ying Lu, Ching-Yu Hsu, Hao-Ting Hsu, Kung-Ming Liu, Wan-Sheng Lo, and Long-Chi Wang 2018/08 Arabidopsis ETO1 and EOL1/2 interact with R2R3-MYB transcription factors,2018植物分子夏令營暨植物學年會(Taiwan Society of Plant Biologists Summer Workshop and Annual Meeting). Tainan走馬瀨農場.
Lin WH, Wang LC, Huang SC, and Lo WS 2018/04 H3K36 demethylase Rph1 modulates the transcriptional reprogramming of cellular quiescence during chronological aging in Saccharomyces cerevisiae.,Cold Spring Harbor Asia Conference: Chromatin, Epigenetics & Transcription.. Worldhotel Grand Dushulake, Suzhou, China..
Islam MT, Wang LC, Chen IJ, Lo KL, and Lo WS 2017/11 Light-mediated seedling development in Arabidopsis thaliana regulated by AtJP1, a H3K4 demethylase.,Taiwan-Japan Plant Biology International Conference (台日植物生物學國際研討會).. Academia Sinica, Taiwan..
Wen-Ju Hsieh, Yi-Ying Lu, Chiao-Mei Cheuh, Wan-Sheng Lo and Long-Chi Wang 2017/11 Potential agonists of ethylene response uncovered by a chemical screening strategy based on yeast two-hybrid system,Meeting report from Taiwan-Japan Plant Biology(台日植物生物學國際研討會). Academia Sinica, Taipei, Taiwan.
Hao-Ting Hsu, Wen-Ju Hsieh, Chung-Yu Chang, Wan-Sheng Lo and Long-Chi Wang 2017/11 Interference with protein-protein interaction of the ETR1-CTR1-EIN2 complex induces ethylene response in Arabidopsis thaliana,Meeting report from Taiwan-Japan Plant Biology(台日植物生物學國際研討會). Academia Sinica, Taipei, Taiwan.
Kung-Ming Liu, Sian-Chi Li, Jhu-Yin Siao, Jui-Cheng Yu, Wan-Sheng Lo and Long-Chi Wang 2017/11 Identification of small molecule inhibitors of Arabidopsis EIN3.,Meeting report from Taiwan-Japan Plant Biology.(台日植物生物學國際研討會). Academia Sinica, Taipei, Taiwan.
Kung-Ming Liou, Shih-Lin Lin, Long-Chi Wang 2016/11 Screening and identification of small chemical molecules interfering with Arabidopsis thaliana EIN3 protein fuction,2016植物學年會 (2016 TSPB Annual Meeting and Symposium). NCHU. Taichung, Taiwan.
Kung-Ming Liou, Shih-Lin Lin, Long-Chi Wang 2016/08 Screening and identification of small chemical molecules interfering with Arabidopsis thaliana EIN3 protein function,2016植物分子生物夏令營(2016 Plant Molecular Biology Summer Camp). Nantou, Taiwan.
Wen-Ju Hsieh, Chun-Yu Chang, Long-Chi Wang 2016 Identification of novel chemical molecules positively regulate ethylene response in Aradibopsis thaliana,2016植物學年會 (2016 TSPB Annual Meeting and Symposium). NCHU, Taichung, Taiwan.
Sian-Chi Li, Long-Chi Wang 2016 Structrul and functional analysis of Arabidopsis ETHYLENE-INSENSITIVE3,2016植物學年會 (2016 TSPB Annual Meeting and Symposium). NCHU, Taichung, Taiwan.
Shin-Lin Lin, Kung-Ming Liou, Long-Chi Wang 2015 Analysis of protein-protein interaction of Oncidium EIN3-Like proteins to design a chemical screening platform for small molecules suppressing ethylene response,台灣植物學年會(2015 TSPB Annual Meeting and Symposium). Academia Sinica, Taipei, Taiwan.